Detection and Identification of Human Parainfluenza Viruses 1, 2, 3, and 4 in Clinical Samples of Pediatric Patients by Multiplex Reverse Transcription-PCR

We describe a multiplex reverse transcription-PCR (m-RT-PCR) assay that is able to detect and differentiate all known human parainfluenza viruses (HPIVs). Serial dilution experiments with reference strains that compared cell culture isolation and m-RT-PCR showed sensitivities ranging from 0.0004 50%...

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Pubblicato in:	J Clin Microbiol
Autori principali:	Aguilar, Jose C., Pérez-Breña, María P., García, María L., Cruz, Nieves, Erdman, Dean D., Echevarría, Juan Emilio
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 2000
Soggetti:	Virology
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC86373/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10699020/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.38.3.1191-1195.2000
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