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Novel Diagnostic Algorithm for Identification of Mycobacteria Using Genus-Specific Amplification of the 16S-23S rRNA Gene Spacer and Restriction Endonucleases

A novel genus-specific PCR for mycobacteria with simple identification to the species level by restriction fragment length polymorphism (RFLP) was established using the 16S-23S ribosomal RNA gene (rDNA) spacer as a target. Panspecificity of primers was demonstrated on the genus level by testing 811...

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Bibliografiske detaljer
Main Authors: Roth, Andreas, Reischl, Udo, Streubel, Anna, Naumann, Ludmila, Kroppenstedt, Reiner M., Habicht, Marion, Fischer, Marga, Mauch, Harald
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2000
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC86348/
https://ncbi.nlm.nih.gov/pubmed/10699003
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