Novel Diagnostic Algorithm for Identification of Mycobacteria Using Genus-Specific Amplification of the 16S-23S rRNA Gene Spacer and Restriction Endonucleases

A novel genus-specific PCR for mycobacteria with simple identification to the species level by restriction fragment length polymorphism (RFLP) was established using the 16S-23S ribosomal RNA gene (rDNA) spacer as a target. Panspecificity of primers was demonstrated on the genus level by testing 811...

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הוצא לאור ב:	J Clin Microbiol
Principais autores:	Roth, Andreas, Reischl, Udo, Streubel, Anna, Naumann, Ludmila, Kroppenstedt, Reiner M., Habicht, Marion, Fischer, Marga, Mauch, Harald
פורמט:	Artigo
שפה:	Inglês
יצא לאור:	American Society for Microbiology (ASM) 2000
נושאים:	Mycobacteriology and Aerobic Actinomycetes
גישה מקוונת:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC86348/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10699003/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.38.3.1094-1104.2000
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