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Diversity within the VP4 Gene of Rotavirus P[8] Strains: Implications for Reverse Transcription-PCR Genotyping

A degenerate version (1T1-D) of the rotavirus P[8]-specific primer (1T-1) allowed strains previously untypeable due to the accumulation of point mutations at the primer binding site to be P typed by reverse transcription-PCR. Sequencing of the cDNA followed by sequence alignment and phylogenetic ana...

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Bibliografiska uppgifter
I publikationen:J Clin Microbiol
Huvudupphov: Iturriza-Gómara, Miren, Green, Jon, Brown, David W. G., Desselberger, Ulrich, Gray, James J.
Materialtyp: Artigo
Språk:Inglês
Utgiven: American Society for Microbiology (ASM) 2000
Ämnen:
Länkar:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC86240/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10655412/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.38.2.898-901.2000
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