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Diversity within the VP4 Gene of Rotavirus P[8] Strains: Implications for Reverse Transcription-PCR Genotyping

A degenerate version (1T1-D) of the rotavirus P[8]-specific primer (1T-1) allowed strains previously untypeable due to the accumulation of point mutations at the primer binding site to be P typed by reverse transcription-PCR. Sequencing of the cDNA followed by sequence alignment and phylogenetic ana...

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Bibliographic Details
Main Authors: Iturriza-Gómara, Miren, Green, Jon, Brown, David W. G., Desselberger, Ulrich, Gray, James J.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2000
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC86240/
https://ncbi.nlm.nih.gov/pubmed/10655412
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