Quantification of Fungal DNA by Using Fluorescence Resonance Energy Transfer and the Light Cycler System

The Light Cycler technique combines rapid in vitro amplification of DNA in glass capillaries with real-time species determination and quantification of DNA load. We have established a quantitative PCR protocol for two clinically important pathogens, Candida albicans and Aspergillus fumigatus. The se...

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Publié dans:	J Clin Microbiol
Auteurs principaux:	Loeffler, Juergen, Henke, Norbert, Hebart, Holger, Schmidt, Diethard, Hagmeyer, Lars, Schumacher, Ulrike, Einsele, Hermann
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 2000
Sujets:	Mycology
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC86153/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10655350/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.38.2.586-590.2000
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