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Quantification of Fungal DNA by Using Fluorescence Resonance Energy Transfer and the Light Cycler System

The Light Cycler technique combines rapid in vitro amplification of DNA in glass capillaries with real-time species determination and quantification of DNA load. We have established a quantitative PCR protocol for two clinically important pathogens, Candida albicans and Aspergillus fumigatus. The se...

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Main Authors: Loeffler, Juergen, Henke, Norbert, Hebart, Holger, Schmidt, Diethard, Hagmeyer, Lars, Schumacher, Ulrike, Einsele, Hermann
格式: Artigo
語言:Inglês
出版: American Society for Microbiology 2000
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC86153/
https://ncbi.nlm.nih.gov/pubmed/10655350
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