Elimination of Bacterial DNA from Taq DNA Polymerases by Restriction Endonuclease Digestion
The incidence of false positives due to the presence of bacterial DNA in Taq DNA polymerase is an obstacle to the use of PCR in the diagnosis of infection. We describe a method that uses a restriction enzyme to destroy the ability of contaminating sequences to act as templates for a nested PCR which...
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| Udgivet i: | J Clin Microbiol |
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| Principais autores: | , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology (ASM)
1999
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC85583/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10488219/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.37.10.3402-3404.1999 |
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