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Elimination of Bacterial DNA from Taq DNA Polymerases by Restriction Endonuclease Digestion

The incidence of false positives due to the presence of bacterial DNA in Taq DNA polymerase is an obstacle to the use of PCR in the diagnosis of infection. We describe a method that uses a restriction enzyme to destroy the ability of contaminating sequences to act as templates for a nested PCR which...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Carroll, Nora M., Adamson, Peter, Okhravi, Narciss
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: American Society for Microbiology 1999
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC85583/
https://ncbi.nlm.nih.gov/pubmed/10488219
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