A Highly Sensitive Assay for Detection and Quantitation of Human Cytomegalovirus DNA in Serum and Plasma by PCR and Electrochemiluminescence

We describe a diagnostic PCR assay (D-PCR) and a quantitative PCR assay (Q-PCR) for the detection of human cytomegalovirus (CMV) in plasma and serum. In the D-PCR, DNA was purified from plasma or serum together with internal control (IC) DNA, which monitored both DNA extraction efficiency and PCR ef...

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Detalhes bibliográficos

Publicado no:	J Clin Microbiol
Principais autores:	Boom, René, Sol, Cees, Weel, Jan, Gerrits, Yvette, de Boer, Monique, Wertheim-van Dillen, Pauline
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1999
Assuntos:	Virology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC84811/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10203511/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.37.5.1489-1497.1999
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