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A Highly Sensitive Assay for Detection and Quantitation of Human Cytomegalovirus DNA in Serum and Plasma by PCR and Electrochemiluminescence

We describe a diagnostic PCR assay (D-PCR) and a quantitative PCR assay (Q-PCR) for the detection of human cytomegalovirus (CMV) in plasma and serum. In the D-PCR, DNA was purified from plasma or serum together with internal control (IC) DNA, which monitored both DNA extraction efficiency and PCR ef...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Boom, René, Sol, Cees, Weel, Jan, Gerrits, Yvette, de Boer, Monique, Wertheim-van Dillen, Pauline
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 1999
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC84811/
https://ncbi.nlm.nih.gov/pubmed/10203511
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