Mutations Altering the Predicted Secondary Structure of a Chloroplast 5′ Untranslated Region Affect Its Physical and Biochemical Properties as Well as Its Ability To Promote Translation of Reporter mRNAs Both in the Chlamydomonas reinhardtii Chloroplast and in Escherichia coli

Random mutations were generated in the sequence for the 5′ untranslated region (5′UTR) of the Chlamydomonas reinhardtii chloroplast rps7 mRNA by PCR, the coding sequence for the mutant leaders fused upstream of the lacZ′ reporter in pUC18, and transformed into Escherichia coli, and white colonies we...

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Podrobná bibliografie

Vydáno v:	Mol Cell Biol
Hlavní autoři:	Fargo, David C., Boynton, John E., Gillham, Nicholas W.
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Taylor & Francis 1999
Témata:	Cell and Organelle Structure and Assembly
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC84693/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10490635/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.19.10.6980
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