Mutations Altering the Predicted Secondary Structure of a Chloroplast 5′ Untranslated Region Affect Its Physical and Biochemical Properties as Well as Its Ability To Promote Translation of Reporter mRNAs Both in the Chlamydomonas reinhardtii Chloroplast and in Escherichia coli

Random mutations were generated in the sequence for the 5′ untranslated region (5′UTR) of the Chlamydomonas reinhardtii chloroplast rps7 mRNA by PCR, the coding sequence for the mutant leaders fused upstream of the lacZ′ reporter in pUC18, and transformed into Escherichia coli, and white colonies we...

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Bibliografiska uppgifter
Huvudupphovsmän: Fargo, David C., Boynton, John E., Gillham, Nicholas W.
Materialtyp: Artigo
Språk:Inglês
Publicerad: American Society for Microbiology 1999
Ämnen:
Cell and Organelle Structure and Assembly
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC84693/
https://ncbi.nlm.nih.gov/pubmed/10490635
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