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Enzymatic Synthesis and Fractionation of Fluorescent PolyU RNAs

The physical properties of viral-length polyuridine (PolyU) RNAs, which cannot base-pair and form secondary structures, are compared with those of normal-composition RNAs, composed of comparable numbers of each of A, U, G and C nucleobases. In this protocol, we describe how to synthesize fluorescent...

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Publicat a:Bio Protoc
Autors principals: Beren, Christian, Liu, Katherine N., Dreesens, Lisa L., Knobler, Charles M., Gelbart, William M.
Format: Artigo
Idioma:Inglês
Publicat: Bio-Protocol 2018
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC8328661/
https://ncbi.nlm.nih.gov/pubmed/34395788
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2988
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