Production and purification of homogenous recombinant human selenoproteins reveals a unique codon skipping event in E. coli and GPX4-specific affinity to bromosulfophthalein

Selenoproteins are translated via animal domain-specific elongation machineries that redefine dedicated UGA opal codons from termination of translation to selenocysteine (Sec) insertion, utilizing specific tRNA species and Sec-specific elongation factors. This has made recombinant production of mamm...

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Veröffentlicht in:Redox Biol
Hauptverfasser: Cheng, Qing, Roveri, Antonella, Cozza, Giorgio, Bordin, Luciana, Rohn, Isabelle, Schwerdtle, Tanja, Kipp, Anna, Ursini, Fulvio, Maiorino, Matilde, Miotto, Giovanni, Arnér, Elias S.J.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Elsevier 2021
Schlagworte:
Research Paper
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8326192/
https://ncbi.nlm.nih.gov/pubmed/34304108
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.redox.2021.102070
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