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Assessment of escape from X chromosome inactivation and gene expression in single human immune cells

X-chromosomal genes escaping from X chromosome inactivation (XCI) in immune cells can contribute to sex-specific differences in immune responses. This protocol describes the specific steps to determine escape from XCI and to simultaneously quantify mRNA expression of multiple genes at the single imm...

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Dades bibliogràfiques
Publicat a:STAR Protoc
Autors principals: Hagen, Sven Hendrik, Hennesen, Jana, Altfeld, Marcus
Format: Artigo
Idioma:Inglês
Publicat: Elsevier 2021
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC8319808/
https://ncbi.nlm.nih.gov/pubmed/34355200
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100641
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