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Assessment of escape from X chromosome inactivation and gene expression in single human immune cells

X-chromosomal genes escaping from X chromosome inactivation (XCI) in immune cells can contribute to sex-specific differences in immune responses. This protocol describes the specific steps to determine escape from XCI and to simultaneously quantify mRNA expression of multiple genes at the single imm...

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Bibliografiske detaljer
Udgivet i:STAR Protoc
Main Authors: Hagen, Sven Hendrik, Hennesen, Jana, Altfeld, Marcus
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2021
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8319808/
https://ncbi.nlm.nih.gov/pubmed/34355200
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100641
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