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Protocol for cell preparation and gene delivery in HEK293T and C2C12 cells
Exogenous overexpression of target genes in both general and specific cell types is important for mechanistic studies of gene function. Here, we provide a step-by-step protocol for cell culture, plasmid transfection in HEK293T, and adenoviral infection in C2C12 cells for gene overexpression in vitro...
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| Veröffentlicht in: | STAR Protoc |
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| Hauptverfasser: | , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Elsevier
2021
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8313743/ https://ncbi.nlm.nih.gov/pubmed/34337441 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100497 |
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