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Calcein Binding to Assess Mineralization in Hydrogel Microspheres
We describe a method to assess mineralization by osteoblasts within microspheres using calcein. Fluorescence imaging of calcein bound to the calcium in hydroxyapatite permits assessment of the mineralized portion of the extracellular matrix. Colorimetric imaging of Alizarin Red S complexed with calc...
Gardado en:
| Publicado en: | Polymers (Basel) |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
MDPI
2021
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8309385/ https://ncbi.nlm.nih.gov/pubmed/34301032 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/polym13142274 |
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