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Calcein Binding to Assess Mineralization in Hydrogel Microspheres

We describe a method to assess mineralization by osteoblasts within microspheres using calcein. Fluorescence imaging of calcein bound to the calcium in hydroxyapatite permits assessment of the mineralized portion of the extracellular matrix. Colorimetric imaging of Alizarin Red S complexed with calc...

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Detalles Bibliográficos
Publicado en:Polymers (Basel)
Autores principales: White, Kristopher, Chalaby, Rabab, Lowe, Gina, Berlin, Jacob, Glackin, Carlotta, Olabisi, Ronke
Formato: Artigo
Lenguaje:Inglês
Publicado: MDPI 2021
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC8309385/
https://ncbi.nlm.nih.gov/pubmed/34301032
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/polym13142274
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