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Estimating Real-Time qPCR Amplification Efficiency from Single-Reaction Data

Methods for estimating the qPCR amplification efficiency E from data for single reactions are tested on six multireplicate datasets, with emphasis on their performance as a function of the range of cycles n(1)–n(2) included in the analysis. The two-parameter exponential growth (EG) model that has be...

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Bibliographische Detailangaben
Veröffentlicht in:Life (Basel)
1. Verfasser: Tellinghuisen, Joel
Format: Artigo
Sprache:Inglês
Veröffentlicht: MDPI 2021
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8303528/
https://ncbi.nlm.nih.gov/pubmed/34357065
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/life11070693
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