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In Vitro Reconstitution of Yeast Translation System Capable of Synthesizing Long Polypeptide and Recapitulating Programmed Ribosome Stalling

The rates of translation elongation or termination in eukaryotes are modulated through cooperative molecular interactions involving mRNA, the ribosome, aminoacyl- and nascent polypeptidyl-tRNAs, and translation factors. To investigate the molecular mechanisms underlying these processes, we developed...

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Detalhes bibliográficos
Publicado no:Methods Protoc
Main Authors: Nagai, Riku, Xu, Yichen, Liu, Chang, Shimabukuro, Ayaka, Takeuchi-Tomita, Nono
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8293373/
https://ncbi.nlm.nih.gov/pubmed/34287320
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/mps4030045
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