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Single-molecule Fluorescence Technique to Monitor the Co-transcriptional Formation of G-quadruplex and R-loop Structures

G-quadruplexes (GQ) and R-loops are non-canonical nucleic acid structures related to gene regulation and genome instability that can be formed during transcription; however, their formation mechanisms remain elusive. To address this question, we developed a single-molecule fluorescence technique to...

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Detalles Bibliográficos
Publicado en:Bio Protoc
Main Authors: Lim, Gunhyoung, Hohng, Sungchul
Formato: Artigo
Idioma:Inglês
Publicado: Bio-Protocol 2021
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC8292125/
https://ncbi.nlm.nih.gov/pubmed/34327266
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.4069
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