Protocol for resolving enzyme orientation and dynamics in advanced porous materials via SDSL-EPR

Enzyme encapsulation in metal-organic frameworks (MOFs)/covalent-organic frameworks (COFs) provides advancement in biocatalysis, yet the structural basis underlying the catalytic performance is challenging to probe. Here, we present an effective protocol to determine the orientation and dynamics of...

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Enregistré dans:
Détails bibliographiques
Publié dans:STAR Protoc
Auteurs principaux: Pan, Yanxiong, Li, Hui, Li, Qiaobin, Lenertz, Mary, Schuster, Isabelle, Jordahl, Drew, Zhu, Xiao, Chen, Bingcan, Yang, Zhongyu
Format: Artigo
Langue:Inglês
Publié: Elsevier 2021
Sujets:
Protocol
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC8287244/
https://ncbi.nlm.nih.gov/pubmed/34308381
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100676
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