Protocol for resolving enzyme orientation and dynamics in advanced porous materials via SDSL-EPR

Enzyme encapsulation in metal-organic frameworks (MOFs)/covalent-organic frameworks (COFs) provides advancement in biocatalysis, yet the structural basis underlying the catalytic performance is challenging to probe. Here, we present an effective protocol to determine the orientation and dynamics of...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Pan, Yanxiong, Li, Hui, Li, Qiaobin, Lenertz, Mary, Schuster, Isabelle, Jordahl, Drew, Zhu, Xiao, Chen, Bingcan, Yang, Zhongyu
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2021
Assuntos:
Protocol
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8287244/
https://ncbi.nlm.nih.gov/pubmed/34308381
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100676
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