Predictive approaches to guide the expression of recombinant vaccine targets in Escherichia coli: a case study presentation utilising Absynth Biologics Ltd. proprietary Clostridium difficile vaccine antigens

Bacterial expression systems remain a widely used host for recombinant protein production. However, overexpression of recombinant target proteins in bacterial systems such as Escherichia coli can result in poor solubility and the formation of insoluble aggregates. As a consequence, numerous strategi...

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Dades bibliogràfiques
Publicat a:Appl Microbiol Biotechnol
Autors principals: Hussain, Hirra, McKenzie, Edward A, Robinson, Andrew M, Gingles, Neill A, Marston, Fiona, Warwicker, Jim, Dickson, Alan J
Format: Artigo
Idioma:Inglês
Publicat: Springer Berlin Heidelberg 2021
Matèries:
Methods and Protocols
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC8285303/
https://ncbi.nlm.nih.gov/pubmed/34180005
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00253-021-11405-9
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