Predictive approaches to guide the expression of recombinant vaccine targets in Escherichia coli: a case study presentation utilising Absynth Biologics Ltd. proprietary Clostridium difficile vaccine antigens

Bacterial expression systems remain a widely used host for recombinant protein production. However, overexpression of recombinant target proteins in bacterial systems such as Escherichia coli can result in poor solubility and the formation of insoluble aggregates. As a consequence, numerous strategi...

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Bibliografische gegevens
Gepubliceerd in:Appl Microbiol Biotechnol
Hoofdauteurs: Hussain, Hirra, McKenzie, Edward A, Robinson, Andrew M, Gingles, Neill A, Marston, Fiona, Warwicker, Jim, Dickson, Alan J
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Springer Berlin Heidelberg 2021
Onderwerpen:
Methods and Protocols
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8285303/
https://ncbi.nlm.nih.gov/pubmed/34180005
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00253-021-11405-9
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