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Isolation, Transfection, and Long-Term Culture of Adult Mouse and Rat Cardiomyocytes

Ex vivo culture of the adult mammalian cardiomyocytes (CMs) presents the most relevant experimental system for the in vitro study of cardiac biology. Adult mammalian CMs are terminally differentiated cells with minimal proliferative capacity. The post-mitotic state of adult CMs not only restricts ca...

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Bibliographic Details
Published in:J Vis Exp
Main Authors: Alam, Perwez, Maliken, Bryan D., Ivey, Malina J., Jones, Shannon M., Kanisicak, Onur
Format: Artigo
Language:Inglês
Published: 2020
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC8276663/
https://ncbi.nlm.nih.gov/pubmed/33104067
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/61073
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