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An Image-based Assay for High-throughput Analysis of Cell Proliferation and Cell Death of Adherent Cells

In this protocol, we describe a method to monitor cell proliferation and death by live-cell imaging of propidium iodide (PI)-stained adherent mammalian cells. PI is widely used to assess cell death. However, it is usually used in end-point assays. Recently, we implemented the use of PI for real-time...

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Vydáno v:Bio Protoc
Hlavní autoři: Szalai, Paula, Engedal, Nikolai
Médium: Artigo
Jazyk:Inglês
Vydáno: Bio-Protocol 2018
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC8275286/
https://ncbi.nlm.nih.gov/pubmed/34286042
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2835
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