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Optimized protocol for single-molecule RNA FISH to visualize gene expression in S. cerevisiae

Single-molecule RNA fluorescence in situ hybridization (smFISH) allows subcellular visualization, localization, and quantification of endogenous RNA molecules in fixed cells. The spatial and intensity information of each RNA can be used to distinguish mature from nascent transcripts inside each cell...

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Bibliografiske detaljer
Udgivet i:STAR Protoc
Main Authors: Patel, Heta P., Brouwer, Ineke, Lenstra, Tineke L.
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2021
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8264745/
https://ncbi.nlm.nih.gov/pubmed/34278333
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100647
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