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Optimized protocol for single-molecule RNA FISH to visualize gene expression in S. cerevisiae
Single-molecule RNA fluorescence in situ hybridization (smFISH) allows subcellular visualization, localization, and quantification of endogenous RNA molecules in fixed cells. The spatial and intensity information of each RNA can be used to distinguish mature from nascent transcripts inside each cell...
Gorde:
| Argitaratua izan da: | STAR Protoc |
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| Egile Nagusiak: | , , |
| Formatua: | Artigo |
| Hizkuntza: | Inglês |
| Argitaratua: |
Elsevier
2021
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| Gaiak: | |
| Sarrera elektronikoa: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8264745/ https://ncbi.nlm.nih.gov/pubmed/34278333 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100647 |
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