A protocol for qualitative and quantitative measurement of endosomal processing using hot spot analysis

A detailed quantification of antigen processing by endosomal compartments provides important information on the pattern of protein fragmentation. Here, we describe a protocol that combines gradient purified endosomes, incubated with antigens, followed by hot spot analysis of MS/MS-sequenced peptides...

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Udgivet i:STAR Protoc
Main Authors: Clement, Cristina C., Nanaware, Padma P., Stern, Lawrence J., Santambrogio, Laura
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2021
Fag:
Protocol
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8264744/
https://ncbi.nlm.nih.gov/pubmed/34278334
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100648
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