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A protocol for qualitative and quantitative measurement of endosomal processing using hot spot analysis
A detailed quantification of antigen processing by endosomal compartments provides important information on the pattern of protein fragmentation. Here, we describe a protocol that combines gradient purified endosomes, incubated with antigens, followed by hot spot analysis of MS/MS-sequenced peptides...
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| Publicat a: | STAR Protoc |
|---|---|
| Autors principals: | , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Elsevier
2021
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8264744/ https://ncbi.nlm.nih.gov/pubmed/34278334 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100648 |
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