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A protocol for qualitative and quantitative measurement of endosomal processing using hot spot analysis
A detailed quantification of antigen processing by endosomal compartments provides important information on the pattern of protein fragmentation. Here, we describe a protocol that combines gradient purified endosomes, incubated with antigens, followed by hot spot analysis of MS/MS-sequenced peptides...
Αποθηκεύτηκε σε:
| Τόπος έκδοσης: | STAR Protoc |
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| Κύριοι συγγραφείς: | , , , |
| Μορφή: | Artigo |
| Γλώσσα: | Inglês |
| Έκδοση: |
Elsevier
2021
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| Θέματα: | |
| Διαθέσιμο Online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8264744/ https://ncbi.nlm.nih.gov/pubmed/34278334 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100648 |
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