Co-immunoprecipitation and semi-quantitative immunoblotting for the analysis of protein-protein interactions

Co-immunoprecipitation (co-IP) of protein complexes from cell lysates is widely used to study protein-protein interactions. However, establishing robust co-IP assays often involves considerable optimization. Moreover, co-IP results are frequently presented in non-quantitative ways. This protocol pre...

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Bibliografische gegevens
Gepubliceerd in:STAR Protoc
Hoofdauteurs: Burckhardt, Christoph J., Minna, John D., Danuser, Gaudenz
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Elsevier 2021
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Protocol
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8264609/
https://ncbi.nlm.nih.gov/pubmed/34278331
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100644
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