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Fluorescence-based Single-cell Analysis of Whole-mount-stained and Cleared Microtissues and Organoids for High Throughput Screening

Three-dimensional (3D) cell culture, especially in the form of organ-like microtissues (“organoids”), has emerged as a novel tool potentially mimicking human tissue biology more closely than standard two-dimensional culture. Typically, tissue sectioning is the standard method for immunohistochemical...

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Bibliografiske detaljer
Udgivet i:Bio Protoc
Main Authors: Renner, Henrik, Otto, Mandy, Grabos, Martha, Schöler, Hans R., Bruder, Jan M.
Format: Artigo
Sprog:Inglês
Udgivet: Bio-Protocol 2021
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8260262/
https://ncbi.nlm.nih.gov/pubmed/34262994
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.4050
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