Local Deuteration Enables NMR Observation of Methyl Groups in Proteins from Eukaryotic and Cell‐Free Expression Systems

Therapeutically relevant proteins such as GPCRs, antibodies and kinases face clear limitations in NMR studies due to the challenges in site‐specific isotope labeling and deuteration in eukaryotic expression systems. Here we describe an efficient and simple method to observe the methyl groups of leuc...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Angew Chem Int Ed Engl
Main Authors: Dubey, Abhinav, Stoyanov, Nikolay, Viennet, Thibault, Chhabra, Sandeep, Elter, Shantha, Borggräfe, Jan, Viegas, Aldino, Nowak, Radosław P., Burdzhiev, Nikola, Petrov, Ognyan, Fischer, Eric S., Etzkorn, Manuel, Gelev, Vladimir, Arthanari, Haribabu
Format: Artigo
Jezik:Inglês
Izdano: John Wiley and Sons Inc. 2021
Teme:
Communications
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC8251921/
https://ncbi.nlm.nih.gov/pubmed/33768661
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.202016070
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