Analysis of proteostasis during aging with western blot of detergent-soluble and insoluble protein fractions

Defects in protein quality control are the underlying cause of age-related diseases. The western blot analysis of detergent-soluble and insoluble protein fractions has proven useful in identifying interventions that regulate proteostasis. Here, we describe the protocol for such analyses in Drosophil...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Rai, Mamta, Curley, Michelle, Coleman, Zane, Nityanandam, Anjana, Jiao, Jianqin, Graca, Flavia A., Hunt, Liam C., Demontis, Fabio
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2021
Assuntos:
Protocol
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8246638/
https://ncbi.nlm.nih.gov/pubmed/34235493
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100628
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