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CRISPR-Mediated Endogenous Activation of Fibroin Heavy Chain Gene Triggers Cellular Stress Responses in Bombyx mori Embryonic Cells

SIMPLE SUMMARY: Based on a CRISPRa approach, activating endogenous fibroin heavy chain (FibH) gene expression in Bombyx mori embryonic (BmE) cells, which was driven by a combination of the dCas9-VPR (a tripartite activator, composed of VP64, p65, and Rta) and the sgRNA targeting to the promoter of F...

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書誌詳細
出版年:Insects
主要な著者: Hu, Wenbo, Wang, Xiaogang, Ma, Sanyuan, Peng, Zhangchuan, Cao, Yang, Xia, Qingyou
フォーマット: Artigo
言語:Inglês
出版事項: MDPI 2021
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC8231919/
https://ncbi.nlm.nih.gov/pubmed/34199296
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/insects12060552
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