Genome-wide CRISPR screening reveals genes essential for cell viability and resistance to abiotic and biotic stresses in Bombyx mori

High-throughput genetic screens are powerful methods to interrogate gene function on a genome-wide scale and identify genes responsible to certain stresses. Here, we developed a piggyBac strategy to deliver pooled sgRNA libraries stably into cell lines. We used this strategy to conduct a screen base...

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Detalhes bibliográficos
Publicado no:Genome Res
Main Authors: Chang, Jiasong, Wang, Ruolin, Yu, Kai, Zhang, Tong, Chen, Xiaoxu, Liu, Yue, Shi, Run, Wang, Xiaogang, Xia, Qingyou, Ma, Sanyuan
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2020
Assuntos:
Method
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7263191/
https://ncbi.nlm.nih.gov/pubmed/32424075
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.249045.119
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