A protocol for detecting elemental calcium signals (Ca(2+) puffs) in mammalian cells using total internal reflection fluorescence microscopy

This protocol outlines steps to visualize and detect Ca(2+) puffs following photo-liberation of caged inositol-1,4,5-trisphosphate (IP(3)) from HEK-293 cells expressing only the native IP(3)R type 1 receptor using total internal reflection fluorescence (TIRF) microscopy. TIRF microscopy offers high...

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Detalles Bibliográficos
Publicado en:STAR Protoc
Autores principales: Arige, Vikas, Emrich, Scott M., Yoast, Ryan E., Trebak, Mohamed, Yule, David I.
Formato: Artigo
Lenguaje:Inglês
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC8225975/
https://ncbi.nlm.nih.gov/pubmed/34195673
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100618
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