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A protocol for detecting elemental calcium signals (Ca(2+) puffs) in mammalian cells using total internal reflection fluorescence microscopy
This protocol outlines steps to visualize and detect Ca(2+) puffs following photo-liberation of caged inositol-1,4,5-trisphosphate (IP(3)) from HEK-293 cells expressing only the native IP(3)R type 1 receptor using total internal reflection fluorescence (TIRF) microscopy. TIRF microscopy offers high...
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| Pubblicato in: | STAR Protoc |
|---|---|
| Autori principali: | , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Elsevier
2021
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8225975/ https://ncbi.nlm.nih.gov/pubmed/34195673 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100618 |
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