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Improved pyrrolysine biosynthesis through phage assisted non-continuous directed evolution of the complete pathway

Pyrrolysine (Pyl, O) exists in nature as the 22(nd) proteinogenic amino acid. Despite being a fundamental building block of proteins, studies of Pyl have been hindered by the difficulty and inefficiency of both its chemical and biological syntheses. Here, we improve Pyl biosynthesis via rational eng...

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Bibliografiske detaljer
Udgivet i:Nat Commun
Main Authors: Ho, Joanne M. L., Miller, Corwin A., Smith, Kathryn A., Mattia, Jacob R., Bennett, Matthew R.
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group UK 2021
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Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8225853/
https://ncbi.nlm.nih.gov/pubmed/34168131
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-021-24183-9
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