Application of CRISPR/Cas9 System for Plasmid Elimination and Bacterial Killing of Bacillus cereus Group Strains

The CRISPR-Cas system has been widely applied in prokaryotic genome editing with its high efficiency and easy operation. We constructed some “scissors plasmids” via using the temperature-sensitive pJOE8999 shuttle plasmid, which carry the different 20nt (N20) guiding the Cas9 nuclease as a scissors...

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Bibliografiske detaljer
Udgivet i:Front Microbiol
Main Authors: Wang, Xiaojing, Lyu, Yufei, Wang, Siya, Zheng, Qingfang, Feng, Erling, Zhu, Li, Pan, Chao, Wang, Shenghou, Wang, Dongshu, Liu, Xiankai, Wang, Hengliang
Format: Artigo
Sprog:Inglês
Udgivet: Frontiers Media S.A. 2021
Fag:
Microbiology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8222586/
https://ncbi.nlm.nih.gov/pubmed/34177818
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2021.536357
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