Application of CRISPR/Cas9 System for Plasmid Elimination and Bacterial Killing of Bacillus cereus Group Strains

The CRISPR-Cas system has been widely applied in prokaryotic genome editing with its high efficiency and easy operation. We constructed some “scissors plasmids” via using the temperature-sensitive pJOE8999 shuttle plasmid, which carry the different 20nt (N20) guiding the Cas9 nuclease as a scissors...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Publicado no:Front Microbiol
Main Authors: Wang, Xiaojing, Lyu, Yufei, Wang, Siya, Zheng, Qingfang, Feng, Erling, Zhu, Li, Pan, Chao, Wang, Shenghou, Wang, Dongshu, Liu, Xiankai, Wang, Hengliang
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2021
Assuntos:
Microbiology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8222586/
https://ncbi.nlm.nih.gov/pubmed/34177818
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2021.536357
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!

Registos relacionados