Highly Efficient Genome Engineering in Bacillus anthracis and Bacillus cereus Using the CRISPR/Cas9 System

Genome editing is an effective tool for the functional examination of bacterial genes and for live attenuated vaccine construction. Here, we report a method to edit the genomic DNA of Bacillus anthracis and Bacillus cereus using the clustered regularly interspaced short palindromic repeats (CRISPR)/...

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Detalhes bibliográficos
Publicado no:Front Microbiol
Main Authors: Wang, Yanchun, Wang, Dongshu, Wang, Xiaojing, Tao, Haoxia, Feng, Erling, Zhu, Li, Pan, Chao, Wang, Bowen, Liu, Chunjie, Liu, Xiankai, Wang, Hengliang
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2019
Assuntos:
Microbiology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6736576/
https://ncbi.nlm.nih.gov/pubmed/31551942
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2019.01932
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