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Protocol for visualizing newly synthesized proteins in primary mouse hepatocytes

Selective identification of newly synthesized proteins is challenging because all proteins, both existing and nascent, have the same amino acid pool and are therefore chemically indistinguishable. L-homopropargylglycine is an amino acid analog of methionine containing an alkyne moiety that can under...

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Dades bibliogràfiques
Publicat a:STAR Protoc
Autors principals: Shen, Yuqian, Liu, Wenhua, Zuo, Jian, Han, Junhai, Zhang, Zi Chao
Format: Artigo
Idioma:Inglês
Publicat: Elsevier 2021
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC8220402/
https://ncbi.nlm.nih.gov/pubmed/34189479
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100616
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