Genome-wide mapping of DNA double-strand breaks from eukaryotic cell cultures using Break-seq

We describe a genome-wide DNA double-strand break (DSB) mapping technique, Break-seq. In this protocol, we provide step-by-step instructions for cell embedment in agarose, in-gel DSB labeling and subsequent capture, followed by standard Illumina library construction and sequencing. We also provide t...

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Bibliografiske detaljer
Udgivet i:STAR Protoc
Main Authors: Joshi, Ishita, DeRycke, Jenna, Palmowski, Megan, LeSuer, Robert, Feng, Wenyi
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2021
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Protocol
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8220103/
https://ncbi.nlm.nih.gov/pubmed/34189468
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100554
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