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Genome-wide mapping of DNA double-strand breaks from eukaryotic cell cultures using Break-seq
We describe a genome-wide DNA double-strand break (DSB) mapping technique, Break-seq. In this protocol, we provide step-by-step instructions for cell embedment in agarose, in-gel DSB labeling and subsequent capture, followed by standard Illumina library construction and sequencing. We also provide t...
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| Pubblicato in: | STAR Protoc |
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| Autori principali: | , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Elsevier
2021
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8220103/ https://ncbi.nlm.nih.gov/pubmed/34189468 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100554 |
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