Comparison of SARS-CoV-2 N gene real-time RT-PCR targets and commercially available mastermixes

BACKGROUND: This study aimed to evaluate the impact of four different reverse transcription quantitative PCR (RT-qPCR) master mixes on the performance of SARS-CoV-2 diagnostic PCRs using three primer/probe assays targeting the N gene (A, B and C). The dynamic range and lowest detected quantity was d...

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Bibliografiske detaljer
Udgivet i:J Virol Methods
Main Authors: Brown, Julianne R., O’Sullivan, Denise M., Shah, Divya, Atkinson, Laura, Pereira, Rui P.A., Whale, Alexandra S., Busby, Eloise J., Huggett, Jim F., Harris, Kathryn
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier B.V. 2021
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8215874/
https://ncbi.nlm.nih.gov/pubmed/34166701
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2021.114215
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