Obtaining purified human intestinal epithelia for single-cell analysis and organoid culture

Here, we describe protocols for the preparation and dissociation of human fetal and pediatric intestinal tissue to a high-viability epithelial single-cell suspension. This epithelium-enriched single-cell suspension can then be used to generate single-cell RNA sequencing data as well as to create hum...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Ross, Alexander D.B., Perrone, Francesca, Elmentaite, Rasa, Teichmann, Sarah A., Zilbauer, Matthias
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2021
Assuntos:
Protocol
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8209738/
https://ncbi.nlm.nih.gov/pubmed/34169291
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100597
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