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Protocol for clickable photoaffinity labeling and quantitative chemical proteomics
Here, we describe a protocol for a photoaffinity labeling probe strategy for target deconvolution in live cells. We made a chemical probe by incorporation of a photoreactive group to covalently cross-link with adjacent amino acid residues upon UV irradiation. Click chemistry-based enrichment capture...
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| Publicat a: | STAR Protoc |
|---|---|
| Autors principals: | , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Elsevier
2021
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8209655/ https://ncbi.nlm.nih.gov/pubmed/34169287 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100593 |
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