Generation of versatile ss-dsDNA hybrid substrates for single-molecule analysis

Here, we describe a rapid and versatile protocol to generate gapped DNA substrates for single-molecule (SM) analysis using optical tweezers via site-specific Cas9 nicking and force-induced melting. We provide examples of single-stranded (ss) DNA gaps of different length and position. We outline prot...

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Bibliographic Details
Published in:STAR Protoc
Main Authors: Belan, Ondrej, Moore, George, Kaczmarczyk, Artur, Newton, Matthew D., Anand, Roopesh, Boulton, Simon J., Rueda, David S.
Format: Artigo
Language:Inglês
Published: Elsevier 2021
Subjects:
Protocol
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC8209646/
https://ncbi.nlm.nih.gov/pubmed/34169285
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100588
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