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Split NanoLuc technology allows quantitation of interactions between PII protein and its receptors with unprecedented sensitivity and reveals transient interactions

PII proteins constitute a widespread signal transduction superfamily in the prokaryotic world. The canonical PII signal proteins sense metabolic state of the cells by binding the metabolite molecules ATP, ADP and 2-oxoglutarate. Depending on bound effector molecule, PII proteins interact with and mo...

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Dettagli Bibliografici
Pubblicato in:Sci Rep
Autori principali: Rozbeh, Rokhsareh, Forchhammer, Karl
Natura: Artigo
Lingua:Inglês
Pubblicazione: Nature Publishing Group UK 2021
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC8206089/
https://ncbi.nlm.nih.gov/pubmed/34131190
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-021-91856-2
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